Regulatory

Part:BBa_M36844:Design

Designed by: Jasmine Johnson, Katie Plummer, Matthew Ryan Frankel   Group: Stanford BIOE44 - S11   (2015-10-25)


p14ARF enhancer and promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 474
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 474
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part requires insertion into a plasmid that expresses GFP or another fluorescent protein to allow for detection of transcription.


Source

Homo sapiens p14ARF enhancer sequence source: http://www.nature.com/nature/journal/v440/n7084/full/nature04585.html p14ARF promoter sequence source: http://chromium.liacs.nl/lovd/refseq/CDKN2A_upstream.html#p14

References